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|The cells were digested by 0.02%EDTA from culture dishes. Added cells in coverslips cautionally. Added 30 uL of 4% Paraformaldehyde to fix the dried cells for 30 min at room-temperature, then washed them with dH 2 O for 5 min; added 30 uL of primary antibody(rabbit anti-human K-ras, Boshide Co.) in a 1:100 dilution with PBS to the cells, incubated them in a humidified chamber for 90 min at room temperature, then washed three times with PBS for 5 min, added 30 uL 0f second antibody with FITC( goal anti-rabbit IgM, Proteintech Group Inc. USA) in a 1:100 dilution with PBS then incubated them in a humidified chamber for 2 h at room temperature; washed with PBS for 5 min again, observed the cells through fluorescence microscope and recorded them||The cells were digested by 0.02% EDTA from culture dishes and.
|As the figure2, shown, the K1/siRNA and K2/siRNA just made expression of K-ras mRNA and protein decreased but not disappear, the siRNAs could be provided effective if they made expression decreased over 50 percent  . The reasons they could not inhibit expression completely may be as follow: (1) Intracellular expression of the transfected PCR products had the problem about transfection efficiency; (2) Cells are in the process of persistent division and siRNA could not transfect into new born cells. K3/siRNA had no effect on expression of K-ras and cells. It administrated that some siRNA sequences designed with the exact principle were not effective. It may be associated with genome position siRNA located, which be influenced by genome secondary structure interacted with protein  ; on the other hand, efficient RNAi requires a perfect match of 21 bases between the interfering RNA and its target sequence||As
| ||In an arbitration case concerning the fright disputes arising from a chartparty, the Arbitration Commission sent the arbitration notices on date of 29 May 1998 to the both parties to request that each of them should choose an arbitrator, and the two parties should jointly choose a presiding arbitrator within 20 days from the date they receive the notices. The claimant chose an arbitrator within the limited time, while the respondent failed until the thirtieth of June 1998 . The chairman of the Commission thus appointed an arbitrator for the respondent in accordance with the regulations of the Rules. Then the chairman of the Commission appointed a presiding arbitrator for the two parties according to the Rules. Thus the 3 arbitrators formed a tribunal to start reviewing the case. The respondent' waiving its right to select arbitrator couldn't delay the time of tribunal composition.||In an arbitration case concerning the
| ||In a case of dispute over damage to cargo and discharge fee on M/V "No1 An Ping", the shipowner (claimant) submitted the arbitration application to C MAC in light of the arbitration clause in the chartparty on 26 January 2000 . The ship owner claimed amount of US Dollars 219514 over cargo damages and discharge fee and the interest. The chartererer (respondent) neither chose the arbitrator nor submitted its defense in writing within the limit time in accordance with the Arbitration Rules. Moreover, the respondent even refused to receive the arbitration documents. The Arbitration Commission requested the ship owner's permission, and then sent the arbitration documents to the charterer's last address supplied by the shipowner by the service way. Therefore the collegial tribunal was formed on 5 April 2000 and the tribunal held the oral hearing in Shanghai on June 14 2000 . The charterer did not attend the hearing. The tribunal had an oral haring and noticed the information to the charter after the hearing, offering enough time to the charterer for commenting the hearing. But the charterer still had no reply. The tribunal issued an award by default according to the written materials and the statements at the oral hearing after discussion on 5 September 2000 . The final award decided that the charterer should pay the ship owner Belgium France 2.2 million over the cargo damage under the 4 B/ L, and U.K. Pound 20 thousand over the discharge fee under the 4 B/L, plus the damage over the two items interest at the rate of 7percent annul. The charterer should pay 60 % of the arbitration fee. The award was inforced by the court at last.||In a case of dispute over damage to cargo and discharge fee on M/V "No1 An Ping", the
Since time immmorial, there exists a conflict among the forces of truth and evil. Human beings are often caught between the goodness of judge, and true and the evils of ignorant, cruelty and falsehood. In this ever existing struggle, every one tries to catch power by wealths, material goods, possessions and lands. Thus, evil try to capture the world.
From time and time, God send prophets to free humans from the claws of evil and oppress. Qur'an talks about how these prophets, guided people to true, brotherhood and justice. The prophets - Nuh, Hud, Salih, Ibrahim, Shu'ayb, Musa, 'Isa, Muhammad and others were simple, humble men who struggle against social evils.
This books discusses the life, struggles, sacrifices and achievements of prophets who appeared in past times to guide human beings towards God.
|Since time immemorial, there exists a conflict
This books discusses the li